Anti-DDIT3 antibody [JM10-31]
cat.: ET1703-05
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JM10-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human DDIT3.
Positive control: LOVO cell lysate, PC-12 cell lysate, human breast carcinoma tissue, human stomach carcinoma tissue, human pancreas tissue, mouse pancreas tissue, mouse brain tissue, Hela.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  FC
  IHC-P

1:500-1:1,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P35638 Human | P35639 Mouse | Q62857 Rat
Alternative names: C/EBP homologous protein antibody C/EBP Homology Protein antibody C/EBP zeta antibody C/EBP-homologous protein 10 antibody C/EBP-homologous protein antibody CCAAT/enhancer binding protein homologous protein antibody CEBPZ antibody CHOP 10 antibody CHOP antibody CHOP-10 antibody CHOP10 antibody DDIT 3 antibody DDIT-3 antibody Ddit3 antibody DDIT3_HUMAN antibody DNA Damage Inducible Transcript 3 antibody DNA damage-inducible transcript 3 protein antibody GADD 153 antibody GADD153 antibody Growth Arrest and DNA Damage Inducible Protein 153 antibody Growth arrest and DNA damage inducible protein GADD153 antibody Growth arrest and DNA damage-inducible protein GADD153 antibody MGC4154 antibody
Images
ET1703-05_1.jpg Fig1: Western blot analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: LOVO cell lysate
Lane 2: PC-12 cell lysate
ET1703-05_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-05_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-05_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-05_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-05_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-05, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-05_7.jpg Fig7: Flow cytometric analysis of DDIT3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-05, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.