ADAM17 Recombinant Rabbit Monoclonal Antibody [JM10-35]
cat.: ET1703-06
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, FC, IP, IHC-Fr
Clonality: Monoclonal
Clone number: JM10-35
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 93 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ADAM17 aa 757-790 / 824.
Positive control: SW480 cell lysate, HepG2 cell lysate, HepG2, SKOV-3, SW480, Hela.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  FC
  IF-Cell
  IF-Tissue
  IP
  IHC-Fr

1:500
1:50-1:100
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
1:100
Uniprot #: SwissProt: P78536 Human | Q9Z0F8 Mouse | Q9Z1K9 Rat
Alternative names: A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) A disintegrin and metalloproteinase domain 17 ADA17_HUMAN ADAM 17 ADAM metallopeptidase domain 17 ADAM17 ADAM17 protein CD 156b CD156b CD156b antigen CSVP Disintegrin and metalloproteinase domain-containing protein 17 MGC71942 NISBD NISBD1 Snake venom like protease Snake venom-like protease TACE TNF alpha convertase TNF alpha converting enzyme TNF-alpha convertase TNF-alpha-converting enzyme Tumor Necrosis Factor Alpha Converting Enzyme
Images
ET1703-06_1.jpg Fig1: Western blot analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SW480 cell lysate
Lane 2: HepG2 cell lysate
ET1703-06_2.jpg Fig2: ICC staining of ADAM17 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-06_3.jpg Fig3: ICC staining of ADAM17 in SKOV-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-06_4.jpg Fig4: ICC staining of ADAM17 in SW480 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-06_5.jpg Fig5: Flow cytometric analysis of ADAM17 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-06, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1703-06_6.jpg Fig6: Immunofluorescence analysis of frozen mouse hippocampus tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody (ET1703-06).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-06, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
ET1703-06_7.jpg Fig7: Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody (ET1703-06).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-06, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.