ADAM17 Recombinant Rabbit Monoclonal Antibody [JM10-35]
cat.: ET1703-06
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, FC, IP, IHC-Fr, IHC-P
Clonality: Monoclonal
Clone number: JM10-35
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 93 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ADAM17 aa 757-790 / 824.
Positive control: SW480 cell lysate, HepG2 cell lysate, HepG2, SKOV-3, SW480, Hela, mouse cerebral cortex tissue, mouse hippocampus tissue, rat hippocampus tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  FC
  IF-Cell
  IF-Tissue
  IP
  IHC-Fr
  IHC-P
1:500
1:50-1:100
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
1:100
1:500
Uniprot #: SwissProt: P78536 Human | Q9Z0F8 Mouse | Q9Z1K9 Rat
Alternative names: A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) A disintegrin and metalloproteinase domain 17 ADA17_HUMAN ADAM 17 ADAM metallopeptidase domain 17 ADAM17 ADAM17 protein CD 156b CD156b CD156b antigen CSVP Disintegrin and metalloproteinase domain-containing protein 17 MGC71942 NISBD NISBD1 Snake venom like protease Snake venom-like protease TACE TNF alpha convertase TNF alpha converting enzyme TNF-alpha convertase TNF-alpha-converting enzyme Tumor Necrosis Factor Alpha Converting Enzyme
Images
ET1703-06_1.jpg Fig1: Western blot analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SW480 cell lysate
Lane 2: HepG2 cell lysate
ET1703-06_2.jpg Fig2: ICC staining of ADAM17 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-06_3.jpg Fig3: ICC staining of ADAM17 in SKOV-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-06_4.jpg Fig4: ICC staining of ADAM17 in SW480 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-06, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-06_5.jpg Fig5: Flow cytometric analysis of ADAM17 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-06, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1703-06_6.jpg Fig6: Immunofluorescence analysis of frozen mouse hippocampus tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody (ET1703-06).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-06, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
ET1703-06_7.jpg Fig7: Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody (ET1703-06).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-06, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
ET1703-06_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-ADAM17 antibody (ET1703-06) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-06) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-06_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-ADAM17 antibody (ET1703-06) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-06) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.