Chromogranin A Recombinant Rabbit Monoclonal Antibody [JM103-7]
cat.: ET1703-08
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JM103-7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Chromogranin A aa 1-50 / 457.
Positive control: PC-12 cell lysates, PC-3M, human pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human colon tissue.
Subcellular location: Cytoplasmic vesicle, Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:200-1:1,000
Uniprot #: SwissProt: P10645 Human | P26339 Mouse | P10354 Rat
Alternative names: beta Granin betagranin (N-terminal fragment of chromogranin A) catestatin CgA CHG A Chga chromofungin Chromogranin A parathyroid secretory protein 1 Chromogranin A precursor ChromograninA CMGA_HUMAN ER-37 Pancreastatin Parastatin Parathyroid secretory protein 1 Pituitary secretory protein I Secretory protein I SP I SP-I SP1 SPI Vasostatin Vasostatin I Vasostatin II
Images
ET1703-08_1.jpg Fig1: Western blot analysis of Chromogranin A on PC-12 cell lysates with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 90 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1703-08_2.jpg Fig2: ICC staining Chromogranin A in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-08_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-08) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-08_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-08) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-08_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-08_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-08_7.jpg Fig7: Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-08, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1703-08_8.jpg Fig8: Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ET1703-08) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-08, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.