Anti-IgA antibody [JM10-42]
cat.: ET1703-10
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, IHC-P, IP
Clonality: Monoclonal
Clone number: JM10-42
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 60 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of Human IgA aa 1-353 / 353.
Positive control: Human plasma lysates, HepG2, human tonsil tissue, human spleen tissue.
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  IP

1:1,000
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P01876 Human | P01877 Human
Alternative names: Hepatocellular carcinoma-associated protein TB6 Ig alpha 1 chain C region Ig alpha 2 chain C region IGHA IGHA1 IGHA2 Immunoglobulin heavy constant alpha 1 Immunoglobulin heavy constant alpha 2 A2m marker Immunoglobulin heavy constant alpha 2 PIgR Poly-Ig receptor polymeric immunoglobulin receptor polymeric immunoglobulin receptor Secretory component
Images
ET1703-10_1.jpg Fig1: Western blot analysis of IgA on human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1703-10_2.jpg Fig2: ICC staining of IgA in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-10, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-10_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-10_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.