IgA Recombinant Rabbit Monoclonal Antibody [JM10-42]
cat.: ET1703-10
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JM10-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Human IgA aa 1-353 / 353. |
| Positive control: | Human plasma lysates, HepG2, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01876 Human | P01877 Human |
| Alternative names: | Hepatocellular carcinoma-associated protein TB6 Ig alpha 1 chain C region Ig alpha 2 chain C region IGHA IGHA1 IGHA2 Immunoglobulin heavy constant alpha 1 Immunoglobulin heavy constant alpha 2 A2m marker Immunoglobulin heavy constant alpha 2 PIgR Poly-Ig receptor polymeric immunoglobulin receptor polymeric immunoglobulin receptor Secretory component |
Images
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Fig1:
Western blot analysis of IgA on human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 38 kDa Observed band size: 60 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.