Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody [JM34-10]
cat.: ET1703-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JM34-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Aryl hydrocarbon Receptor aa 735-775 / 848. |
| Positive control: | HepG2 cell lysate, A549 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P35869 Human |
| Alternative names: | Ah receptor AhR AHR_HUMAN Aromatic hydrocarbon receptor Aryl hydrocarbon receptor Aryl hydrocarbon receptor precursor bHLHe76 Class E basic helix loop helix protein 76 Class E basic helix-loop-helix protein 76 HGNC:348 |
Images
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Fig1:
Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody (ET1703-11) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 96 kDa Observed band size: 105 kDa Exposure time: 2 minutes 37 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Aryl hydrocarbon Receptor on different lysates with Rabbit anti-Aryl hydrocarbon Receptor antibody (ET1703-11) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Aryl hydrocarbon Receptor cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 105 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-11) at 1/1,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.