HRP Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [JM10-56]
cat.: ET1703-12
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JM10-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein |
| Positive control: | C-terminal HA-tag recombinant protein. |
| Recommended Dilutions:
WB |
1:2,000 |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
|
Fig1:
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (ET1703-12) at 1/2,000 dilution. Lane 1: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate (10 µg/Lane) Lane 2: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate (5 µg/Lane) Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-12) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.