HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10]
cat.: ET1703-13
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Species independent
Applications: WB
Clonality: Monoclonal
Clone number: JM57-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: recombinant protein
Positive control: 293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate.
Recommended Dilutions:
  WB

1:5,000
Alternative names: Glutathione S Transferase Glutathione S-transferase class-mu 26 kDa isozyme GST
Images
ET1703-13_1.jpg Fig1: Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (ET1703-13) at 1/5,000 dilution.

Lane 1: 293T transfected with GST cell lysate (5 µg/Lane)
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-13) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.