Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Species independent |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JM57-10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Isotype: | IgG |
Immunogen: | recombinant protein |
Positive control: | 293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate. |
Recommended Dilutions:
WB |
1:5,000 |
Alternative names: | Glutathione S Transferase Glutathione S-transferase class-mu 26 kDa isozyme GST |
Fig1:
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (ET1703-13) at 1/5,000 dilution. Lane 1: 293T transfected with GST cell lysate (5 µg/Lane) Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane) Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-13) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |