Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
cat.: ET1703-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JM100-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Paravalbumin aa 1-110 / 110. |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, RPMI 8226 cell lysate, Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Axon, cytoplasm, synapse. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue IP |
1:1,000 1:5,000-1:50,000 1:500-1:1,000 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: P20472 Human | P32848 Mouse | P02625 Rat |
| Alternative names: | D22S749 MGC116759 Parvalbumin alpha PRVA_HUMAN PVALB |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Parvalbumin, ET1703-15, red); 1/500 (SLC32A1 / VGAT, HA601384, green) Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Parvalbumin, ET1703-15, red); 1/500 (SLC32A1 / VGAT, HA601384, green) Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution. Lane 1: RPMI 8226 cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 12 kDa Observed band size: 14 kDa Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig10:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig11:
Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with ET1703-15 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-15 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RPMI 8226 cell lysate (input) Lane 2: ET1703-15 IP in RPMI 8226 cell lysate Lane 3: Rabbit IgG instead of ET1703-15 in RPMI 8226 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.