Interferon gamma Recombinant Rabbit Monoclonal Antibody [JM10-10]
cat.: ET1703-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JM10-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Interferon gamma. |
| Positive control: | Human IFN-gamma recombinant protein, Jurkat. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB FC |
1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P01579 Human |
| Alternative names: | IFG IFI IFN gamma IFN, immune IFN-gamma IFNG IFNG_HUMAN Immune interferon Interferon gamma |
Images
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Fig1:
Western blot analysis of Interferon gamma on different lysates with Rabbit anti-Interferon gamma antibody (ET1703-17) at 1/1,000 dilution. Lane 1: Human IFN-gamma recombinant protein, 50ng/Lane Lane 2: Mouse IFN-gamma recombinant protein, 50ng/Lane Lane 3: Rat IFN-gamma recombinant protein, 50ng/Lane Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-17) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of Interferon gamma was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.