Anti-Interferon gamma antibody [JM10-10]
cat.: ET1703-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JM10-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 19 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Interferon gamma.
Positive control: Human liver tissue, human liver carcinoma tissue, mouse liver tissue, Jurkat.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  FC
  IHC-P

1:500
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P01579 Human | P01580 Mouse | P01581 Rat
Alternative names: IFG antibody IFI antibody IFN gamma antibody IFN, immune antibody IFN-gamma antibody IFNG antibody IFNG_HUMAN antibody Immune interferon antibody Interferon gamma antibody
Images
ET1703-17_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-17_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-17_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-17_4.jpg Fig4: Flow cytometric analysis of Interferon gamma was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.