ERCC1 Recombinant Rabbit Monoclonal Antibody [JM10-07]
cat.: ET1703-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ERCC1 aa 1-220 / 297. |
| Positive control: | HeLa cell lysate, A549 cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Raji cell lysate, Hela, A549, HepG2, PANC-1, 293T, human kidney tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000 1:50 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: P07992 Human | P07903 Mouse |
| Alternative names: | COFS 4 COFS4 DNA excision repair protein ERCC 1 DNA excision repair protein ERCC-1 DNA excision repair protein ERCC1 ERCC 1 ERCC1 ERCC1_HUMAN Excision repair cross complementation group 1 Excision repair cross complementing 1 Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 Excision repair protein RAD 10 RAD10 UV 20 UV20 |
Images
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Fig1:
Western blot analysis of ERCC1 on different lysates with Rabbit anti-ERCC1 antibody (ET1703-19) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SK-Br-3 cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-19) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: ICC staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.