Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, FC |
Clonality: | Monoclonal |
Clone number: | JM101-05 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -81℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 210 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human NOTCH4 aa 1,920-1,960 / 2,003. |
Positive control: | Hela. |
Subcellular location: | Cell membrane, Nucleus. |
Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
Uniprot #: | SwissProt: Q99466 Human | P31695 Mouse |
Alternative names: | FLJ16302 hNotch 4 hNotch4 INT3 Neurogenic locus notch homolog protein 4 NOTC4_HUMAN Notch 4 Notch 4 intracellular domain Notch homolog 4 NOTCH3 Notch4 NOTCH4 protein |
Fig1: Flow cytometric analysis of NOTCH4 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-20, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |