NOTCH4 Recombinant Rabbit Monoclonal Antibody [JM101-05]
cat.: ET1703-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM101-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -81℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 210 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NOTCH4 aa 1,920-1,960 / 2,003. |
| Positive control: | F9 cell lysates. |
| Subcellular location: | Cell membrane, Nucleus. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P31695 Mouse |
| Alternative names: | FLJ16302 hNotch 4 hNotch4 INT3 Neurogenic locus notch homolog protein 4 NOTC4_HUMAN Notch 4 Notch 4 intracellular domain Notch homolog 4 NOTCH3 Notch4 NOTCH4 protein |
Images
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Fig1:
Western blot analysis of NOTCH4 on F9 cell lysates with Rabbit anti-NOTCH4 antibody (ET1703-20) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 210 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
NOTCH4 was immunoprecipitated from 0.2 mg F9 cell lysate with ET1703-20 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1703-20 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: F9 cell lysate (input) Lane 2: ET1703-20 IP in F9 cell lysate Lane 3: Rabbit IgG instead of ET1703-20 in F9 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.