Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79]
cat.: ET1703-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Haptoglobin aa 357-406 / 406. |
| Positive control: | Human serum lysate, Human plasma lysate, human liver tissue, human lung tissue, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P00738 Human |
| Alternative names: | Binding peptide BP Haptoglobin alpha chain Haptoglobin alpha(1S) beta Haptoglobin alpha(2FS) beta Haptoglobin beta chain Haptoglobin, alpha polypeptide Haptoglobin, beta polypeptide HP HP2 ALPHA2 HP2ALPHA2 HPA1S HPT HPT_HUMAN MGC111141 Zonulin |
Images
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Fig1:
Western blot analysis of Haptoglobin on different lysates with Rabbit anti-Haptoglobin antibody (ET1703-24) at 1/1,000 dilution. Lane 1: Human serum lysate Lane 2: Human plasma lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Haptoglobin antibody (ET1703-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Haptoglobin antibody (ET1703-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: ICC staining of Haptoglobin in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.