EDG1 Recombinant Rabbit Monoclonal Antibody [JM10-66]
cat.: ET1703-27
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JM10-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human EDG1 aa 2-51 / 382. |
| Positive control: | SH-SY5Y cell lysate, HepG2 cell lysate, bEnd.3 cell lysate, HepG2, HUVEC, SH-SY5Y, human breast carcinoma tissue, human liver tissue, mouse brain tissue, mouse heart tissue, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC IP |
1:2,000-5,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P21453 Human | O08530 Mouse | P48303 Rat |
| Alternative names: | CD363 CHEDG 1 CHEDG1 D1S3362 ECGF 1 ECGF1 EDG 1 EDG1 endothelial differentiation G protein coupled receptor 1 Endothelial differentiation G-protein coupled receptor 1 Endothelial differentiation sphingolipid G protein coupled receptor 1 FLJ58121 G protein coupled sphingolipid receptor g protein-coupled receptor edg-1 S1P receptor 1 S1P receptor Edg 1 S1P receptor Edg-1 S1P receptor Edg1 S1P(1) receptor S1P1 s1pr1 S1PR1_HUMAN sphingolipid g-protein-coupled receptor 1 Sphingosine 1 phosphate receptor Edg 1 Sphingosine 1 phosphate receptor EDG1 sphingosine 1- phosphate receptor 1 Sphingosine 1-phosphate receptor 1 Sphingosine 1-phosphate receptor Edg-1 Sphingosine 1phosphate receptor type 1 S1P1 |
Images
|
Fig1:
Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (ET1703-27) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate Lane 3: bEnd.3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-27) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (ET1703-27) at 1/5,000 dilution. Lane 1: HepG2-si NT cell lysate Lane 2: HepG2-si EDG1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-27) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of EDG1 on different lysates with Rabbit anti-EDG1 antibody (ET1703-27) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: PC-12 cell lysate Lane 3: Rat brain tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20/40 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-27) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4: Flow cytometric analysis of EDG1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig5:
EDG1 was immunoprecipitated in 0.2mg HepG2 cell lysate with ET1703-27 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1703-27 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: ET1703-27 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of ET1703-27 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 mintues |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.