| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM10-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD59 aa 85-121 / 128. |
| Positive control: | Human placenta tissue lysate, HUVEC cell lysate, K562 cell lysate. |
| Subcellular location: | Cell membrane. Secreted. |
| Recommended Dilutions:
WB IP |
1:1,000-1:5,000 1:10-1:50 |
| Uniprot #: | SwissProt: P13987 Human |
| Alternative names: | 16.3A5 1F5 1F5 antigen 20 kDa homologous restriction factor CD 59 CD_antigen=CD59 CD59 CD59 antigen CD59 antigen complement regulatory protein CD59 antigen p18 20 CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) CD59 glycoprotein CD59 molecule CD59 molecule complement regulatory protein CD59_HUMAN Cd59a Complement regulatory protein EJ16 EJ30 EL32 FLJ38134 FLJ92039 G344 HRF 20 HRF-20 HRF20 Human leukocyte antigen MIC11 Ly 6 like protein Lymphocytic antigen CD59/MEM43 MAC inhibitory protein MAC IP MAC-inhibitory protein MAC-IP MACIF MACIP MEM43 MEM43 antigen Membrane attack complex (MAC) inhibition factor Membrane attack complex inhibition factor Membrane inhibitor of reactive lysis MGC2354 MIC11 MIN1 MIN2 MIN3 MIRL MSK21 p18 20 Protectin Surface antigen recognized by monoclonal 16.3A5 T cell activating protein |
|
Fig1:
Western blot analysis of CD59 on different lysates with Rabbit anti-CD59 antibody (ET1703-28) at 1/10,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1703-28, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 14 kDa Observed band size: 15 kDa |