Anti-CD59 antibody [JM10-71]
cat.: ET1703-28
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC/IF, FC, IP
Clonality: Monoclonal
Clone number: JM10-71
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 14/20 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: NIH-3T3, HUVEC, JAR, K562, Human placenta.
Subcellular location: Cell membrane. Secreted.
Recommended Dilutions:
  WB
  ICC/IF
  FC
  IP

1:1,000-1:5,000
1:100-1:500
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: P13987 Human
Alternative names: 16.3A5 antibody 1F5 antibody 1F5 antigen antibody 20 kDa homologous restriction factor antibody CD 59 antibody CD_antigen=CD59 antibody CD59 antibody CD59 antigen antibody CD59 antigen complement regulatory protein antibody CD59 antigen p18 20 antibody CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody CD59 glycoprotein antibody CD59 molecule antibody CD59 molecule complement regulatory protein antibody CD59_HUMAN antibody Cd59a antibody Complement regulatory protein antibody EJ16 antibody EJ30 antibody EL32 antibody FLJ38134 antibody FLJ92039 antibody G344 antibody HRF 20 antibody HRF-20 antibody HRF20 antibody Human leukocyte antigen MIC11 antibody Ly 6 like protein antibody Lymphocytic antigen CD59/MEM43 antibody MAC inhibitory protein antibody MAC IP antibody MAC-inhibitory protein antibody MAC-IP antibody MACIF antibody MACIP antibody MEM43 antibody MEM43 antigen antibody Membrane attack complex (MAC) inhibition factor antibody Membrane attack complex inhibition factor antibody Membrane inhibitor of reactive lysis antibody MGC2354 antibody MIC11 antibody MIN1 antibody MIN2 antibody MIN3 antibody MIRL antibody MSK21 antibody p18 20 antibody Protectin antibody Surface antigen recognized by monoclonal antibody 16.3A5 antibody T cell activating protein antibody
Images
ET1703-28_1.jpg Fig1: Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution.
Positive control:
Lane 1: Human placenta
Lane 2: HUVEC
Lane 3: K562
ET1703-28_2.jpg Fig2: ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-28_3.jpg Fig3: ICC staining CD59 in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-28_4.jpg Fig4: ICC staining CD59 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-28_5.jpg Fig5: Flow cytometric analysis of NIH-3T3 cells with CD59 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.