Anti-ATPB antibody [JM10-90]
cat.: ET1703-29
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IP, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: JM10-90
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 53 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: 293T, HepG2, A431, Hela, human liver tissue, human kidney tissue, mouse uterus tissue, mouse liver muscle tissue, mouse brain tissue, mouse heart tissue, zebrafish tissue.
Subcellular location: Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  IP

1:1,000-1:5,000
1:100-1:500
1:50-1:200
1:10-1:50
Uniprot #: P06576(Human) P56480(Mouse) P10719(Rat)
Alternative names: ATP 5B antibody ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody ATP synthase subunit beta mitochondrial antibody ATP synthase subunit beta, mitochondrial antibody atp5b antibody ATPB antibody ATPB_HUMAN antibody ATPMB antibody ATPSB antibody Epididymis secretory protein Li 271 antibody HEL-S-271 antibody Mitochondrial ATP synthase beta subunit antibody Mitochondrial ATP Synthase Subunit Beta antibody Mitochondrial ATP synthetase beta subunit antibody
Images
ET1703-29_1.jpg Fig1: Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution.
Positive control: Line1: Hela Line2: HepG2 Line3: 293T
ET1703-29_2.jpg Fig2: ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-29_3.jpg Fig3: ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-29_4.jpg Fig4: ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-29_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ET1703-29_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ET1703-29_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ET1703-29_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ET1703-29_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ET1703-29_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ET1703-29_11.jpg Fig11: Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.