Anti-ATPB antibody [JM10-90]
cat.: ET1703-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IP, ICC/IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | recombinant protein |
| Positive control: | 293T, HepG2, A431, Hela, human liver tissue, human kidney tissue, mouse uterus tissue, mouse liver muscle tissue, mouse brain tissue, mouse heart tissue, zebrafish tissue. |
| Subcellular location: | Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. |
| Recommended Dilutions:
WB ICC/IF IHC-P IP |
1:1,000-1:5,000 1:100-1:500 1:50-1:200 1:10-1:50 |
| Uniprot #: | P06576(Human) P56480(Mouse) P10719(Rat) |
| Alternative names: | ATP 5B antibody ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody ATP synthase subunit beta mitochondrial antibody ATP synthase subunit beta, mitochondrial antibody atp5b antibody ATPB antibody ATPB_HUMAN antibody ATPMB antibody ATPSB antibody Epididymis secretory protein Li 271 antibody HEL-S-271 antibody Mitochondrial ATP synthase beta subunit antibody Mitochondrial ATP Synthase Subunit Beta antibody Mitochondrial ATP synthetase beta subunit antibody |
Images
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Fig1:
Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T |
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Fig2: ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin. |
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Fig10: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin. |
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Fig11: Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.