TLR5 Recombinant Rabbit Monoclonal Antibody [JM10-88]
cat.: ET1703-30
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TLR5 aa 238-424 / 858. |
| Positive control: | SK-Br-3 cells lysates, mouse testis tissue, mouse prostate tissue, mouse stomach tissue, human spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O60602 Human | Q9JLF7 Mouse Unigene: 198962 Rat |
| Alternative names: | FLJ10052 MGC126430 MGC126431 SLEB1 TIL 3 TIL3 TLR 5 Tlr5 TLR5_HUMAN Toll like receptor 5 Toll like receptor 5 precursor Toll-like receptor 5 Toll/interleukin 1 receptor like protein 3 Toll/interleukin-1 receptor-like protein 3 |
Images
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Fig1:
Western blot analysis of TLR5 on SK-Br-3 cell lysates with Rabbit anti-TLR5 antibody (ET1703-30) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-30) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TLR5 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-TLR5 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-TLR5 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR5 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.