Cyclophilin A Recombinant Rabbit Monoclonal Antibody [JM107-03]
cat.: ET1703-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM107-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cyclophilin A aa 131-159 / 165. |
| Positive control: | Hela cell lysate, A431 cell lysate, HepG2 cell lysate, human breast carcinoma tissue, human lung tissue, human pancreas tissue, mouse lung tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P62937 Human | P17742 Mouse | P10111 Rat |
| Alternative names: | Cyclophilin A Cyclophilin CyclophilinA Cyclosporin A binding protein Cyclosporin A-binding protein CYPA CYPH Epididymis secretory sperm binding protein Li 69p HEL S 69p MGC117158 MGC12404 MGC23397 Peptidyl prolyl cis trans isomerase A Peptidyl-prolyl cis-trans isomerase A Peptidylprolyl isomerase A (cyclophilin A) Peptidylprolyl isomerase A PPIA PPIA protein PPIA_HUMAN PPIase A Rotamase A RotamaseA T cell cyclophilin |
Images
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Fig1:
Western blot analysis of Cyclophilin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 3: HepG2 cell lysate Predicted band size: 18 kDa Observed band size: 14 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclophilin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Cyclophilin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Cyclophilin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Cyclophilin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.