SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]
cat.: ET1703-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM10-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 142 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SMC3 aa 1994-1217 / 1217. |
| Positive control: | HepG2 cell lysate, NIH-3T3 cell lysate, PC12 cell lysate, Hela, HepG2, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse colon tissue, zebrafish tissue. |
| Subcellular location: | Centromere, Chromosome, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UQE7 Human | Q9CW03 Mouse | P97690 Rat |
| Alternative names: | BAM Bamacan Basement membrane associated chondroitin proteoglycan Basement membrane-associated chondroitin proteoglycan BMH CDLS3 chondroitin sulfate proteoglycan 6 (bamacan) Chondroitin sulfate proteoglycan 6 Chromosome associated polypeptide Chromosome-associated polypeptide CSPG 6 CSPG6 hCAP Human chromosome associated polypeptide im:7142991 SMC 3 SMC protein 3 SMC-3 smc3 SMC3_HUMAN SMC3L1 Structural maintenance of chromosome 3 Structural maintenance of chromosomes 3 Structural maintenance of chromosomes protein 3 u:fb22e01 wu:fc30d07 |
Images
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Fig1:
Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution. Positive control: Line1: HepG2 cell lysate Line2: NIH-3T3 cell lysate Line3:PC12 cell lysate |
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Fig2: ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.