SMC3 Recombinant Rabbit Monoclonal Antibody [JM10-75]
cat.: ET1703-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM10-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 142 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SMC3 aa 1994-1217 / 1217. |
| Positive control: | HepG2 cell lysate, NIH-3T3 cell lysate, PC12 cell lysate, Hela, HepG2, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse colon tissue, zebrafish tissue. |
| Subcellular location: | Centromere, Chromosome, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UQE7 Human | Q9CW03 Mouse | P97690 Rat |
| Alternative names: | BAM Bamacan Basement membrane associated chondroitin proteoglycan Basement membrane-associated chondroitin proteoglycan BMH CDLS3 chondroitin sulfate proteoglycan 6 (bamacan) Chondroitin sulfate proteoglycan 6 Chromosome associated polypeptide Chromosome-associated polypeptide CSPG 6 CSPG6 hCAP Human chromosome associated polypeptide im:7142991 SMC 3 SMC protein 3 SMC-3 smc3 SMC3_HUMAN SMC3L1 Structural maintenance of chromosome 3 Structural maintenance of chromosomes 3 Structural maintenance of chromosomes protein 3 u:fb22e01 wu:fc30d07 |
Images
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Fig1:
Western blot analysis of SMC3 on HepG2 lysates with Rabbit anti-SMC3 antibody (ET1703-35) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1703-35, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 141.5kDa Observed band size: 142 kDa |
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Fig2: ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.