Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [JM10-67]
cat.: ET1703-39
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Lipocalin-2 aa 50-100/198. |
| Positive control: | A431 cell lysates, human spleen tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P80188 Human |
| Alternative names: | 24p3 25 kDa alpha 2 microglobulin related subunit of MMP9 25 kDa alpha-2-microglobulin-related subunit of MMP-9 Alpha 2 microglobulin related protein HGNC:6526 HNL Lcn 2 Lcn2 Lipocalin-2 Migration stimulating factor inhibitor MSFI Neutrophil gelatinase associated lipocalin Neutrophil gelatinase associated lipocalin precursor Neutrophil gelatinase-associated lipocalin NGAL NGAL_HUMAN Oncogene 24p3 Oncogenic lipocalin 24p3 p25 Siderocalin siderocalin LCN2 SV40 induced 24P3 protein Uterocalin |
Images
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Fig1:
Western blot analysis of Lipocalin-2 on A431 cell lysates with Rabbit anti-Lipocalin-2 antibody (ET1703-39) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Lipocalin-2 antibody (ET1703-39) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-39) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.