Anti-SDHA antibody [JM10-83]
cat.: ET1703-40
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JM10-83
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 68 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SDHA aa 581-624 / 664.
Positive control: Jurkat, MCF-7, HepG2, NIH-3T3, Hela, human liver tissue, human kidney tissue, mouse testis tissue, mouse skeletal muscle tissue, mouse colon tissue, zebrafish tissue.
Subcellular location: Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P31040 Human | Q8K2B3 Mouse | Q920L2 Rat
Alternative names: CMD1GG DHSA_HUMAN Flavoprotein subunit of complex II Fp PGL5 SDH 2 SDH1 SDH2 SDHA SDHF Succinate dehydrogenase [ubiquinone] flavoprotein subunit Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Succinate dehydrogenase complex flavoprotein subunit A Succinate dehydrogenase complex flavoprotein subunit Succinate dehydrogenase complex flavoprotein subunit precursor Succinate dehydrogenase complex subunit A Succinate dehydrogenase complex subunit A flavoprotein (Fp) Succinate dehydrogenase complex subunit A flavoprotein
Images
ET1703-40_1.jpg Fig1: Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.
ET1703-40_2.jpg Fig2: Western blot analysis of SDHA on different cell lysates using anti-SDHA antibody at 1/500 dilution.Positive control:
Lane 1:HepG2
Lane 2:SHG44
ET1703-40_3.jpg Fig3: ICC staining SDHA in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-40_4.jpg Fig4: ICC staining SDHA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-40_5.jpg Fig5: ICC staining SDHA in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-40_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHA antibody. Counter stained with hematoxylin.
ET1703-40_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHA antibody. Counter stained with hematoxylin.
ET1703-40_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SDHA antibody. Counter stained with hematoxylin.
ET1703-40_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SDHA antibody. Counter stained with hematoxylin.
ET1703-40_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHA antibody. Counter stained with hematoxylin.
ET1703-40_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SDHA antibody. Counter stained with hematoxylin.
ET1703-40_12.jpg Fig12: Flow cytometric analysis of Hela cells with SDHA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.