CD32 Recombinant Rabbit Monoclonal Antibody [JM10-70]
cat.: ET1703-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM10-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD32 aa 23-274 / 310. |
| Positive control: | Human lung tissue lysates, human tonsil tissue, human spleen tissue, human pancreas tissue, K-562. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000-1:5,000 1:50-1:400 1:100 |
| Uniprot #: | SwissProt: P12318 Human |
| Alternative names: | CD32 CD32A CD32B CD32C CDw32 Fc fragment of IgG low affinity IIa receptor Fc fragment of IgG low affinity IIa receptor for (CD32) Fc fragment of IgG low affinity IIb receptor Fc fragment of IgG low affinity IIb receptor for (CD32) Fc fragment of IgG low affinity IIc receptor Fc fragment of IgG low affinity IIc receptor for (CD32) Fc fragment of IgG, low affinity II, receptor for (CD32) Fc fragment of IgG, low affinity IIa, receptor (CD32) Fc fragment of IgG, low affinity IIb, receptor (CD32) Fc gamma receptor IIB Fc gamma receptor IIC Fc gamma RII a Fc gamma RII b Fc gamma RII c Fc gamma RIIb Fc gamma RIIc Fc receptor, IgG, low affinity IIb Fc-gamma RII-a Fc-gamma RII-b Fc-gamma RII-c Fc-gamma-RIIa FC-gamma-RIIB Fc-GAMMA-RIIC Fc[g]RII FCG2 FCG2A_HUMAN FcGR FCGR2 FCGR2A FCGR2A1 FCGR2B FCGR2C Fcgr3 Fcgr3a FcgRII Fcr-2 Fcr-3 FcRII a FCRII FcRII b FcRII c FcRII-a FcRIIC IGFR2 IgG Fc receptor I...... |
Images
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Fig1: Western blot analysis of CD32 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD32 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD32 antibody (ET1703-43) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-43) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD32 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of K-562 cells labeling CD32 with Rabbit anti-CD32 antibody (ET1703-43) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD32 antibody (ET1703-43) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.