Anti-Hemoglobin subunit gamma 1and2 antibody [JM84-10]
cat.: ET1703-46
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: JM84-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 16 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human HBG1 / 2 aa 2-45 / 147.
Positive control: Human placenta tissue lysate, human brain tissue lysate, D3, MCF-7, PC-12, human tonsil tissue, human spleen tissue.
Subcellular location: Cytosol, hemoglobin complex.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:1,000-1:5,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P69891 Human | P69892 Human
Alternative names: Hemoglobin subunit gamma-1 Gamma-1-globin Hb F Agamma Hemoglobin gamma-1 chain Hemoglobin gamma-A chain HBG1 PRO2979 Abnormal hemoglobin FLJ76540 G gamma globin Gamma 2 globin Gamma-2-globin Hb F Ggamma HBG 2 HBG2 HBG2_HUMAN Hemoglobin gamma 2 chain Hemoglobin gamma G Hemoglobin gamma G chain Hemoglobin gamma-2 chain Hemoglobin gamma-G chain Hemoglobin subunit gamma 2 Hemoglobin subunit gamma-2 Methemoglobin OTTHUMP00000069638
Images
ET1703-46_1.jpg Fig1: Western blot analysis of Hemoglobin subunit gamma 1and2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human placenta tissue lysate
Lane 2: human brain tissue lysate
ET1703-46_2.jpg Fig2: ICC staining of Hemoglobin subunit gamma 1and2 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-46_3.jpg Fig3: ICC staining of Hemoglobin subunit gamma 1and2 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-46_4.jpg Fig4: ICC staining of Hemoglobin subunit gamma 1and2 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-46_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hemoglobin subunit gamma 1and2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-46_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Hemoglobin subunit gamma 1and2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-46, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.