CYP2E1 Recombinant Rabbit Monoclonal Antibody [JM10-85]
cat.: ET1703-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CYP2E1 aa 71-114 / 439. |
| Positive control: | Human liver tissue lysates, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P05181 Human | Q05421 Mouse | P05182 Rat |
| Alternative names: | 4-nitrophenol 2-hydroxylase CP2E1_HUMAN CPE1 CYP2E Cyp2e1 CYPIIE1 Cytochrome P450 2E1 Cytochrome P450 isozyme 3A Cytochrome P450 LM3A Cytochrome P450, family 2, subfamily e, polypeptide 1 cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 Cytochrome P450, subfamily IIE Cytochrome P450-ALC Cytochrome P450-J Cytochrome P450RLM6 EC 1.14.13.- EC 1.14.13.n7 Ethanol-inducible P450 Flavoprotein-linked monooxygenase MGC133529 Microsomal monooxygenase OTTHUMP00000020813 OTTHUMP00000046571 P450 ALC P450-J P450C2E P450J P450RLM6 Xenobiotic monooxygenase |
Images
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Fig1: Western blot analysis of CYP2E1 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CYP2E1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CYP2E1 antibody (ET1703-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CYP2E1 antibody (ET1703-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CYP2E1 antibody (ET1703-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.