CYP2E1 Recombinant Rabbit Monoclonal Antibody [JM10-85]
cat.: ET1703-48
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JM10-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 57 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CYP2E1 aa 71-114 / 439.
Positive control: Human liver tissue lysates, mouse liver tissue lysate, rat liver tissue lysate, 293T, Hela, human liver carcinoma tissue, human liver tissue, mouse liver tissue.
Subcellular location: Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P05181 Human | Q05421 Mouse | P05182 Rat
Alternative names: 4-nitrophenol 2-hydroxylase CP2E1_HUMAN CPE1 CYP2E Cyp2e1 CYPIIE1 Cytochrome P450 2E1 Cytochrome P450 isozyme 3A Cytochrome P450 LM3A Cytochrome P450, family 2, subfamily e, polypeptide 1 cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 Cytochrome P450, subfamily IIE Cytochrome P450-ALC Cytochrome P450-J Cytochrome P450RLM6 EC 1.14.13.- EC 1.14.13.n7 Ethanol-inducible P450 Flavoprotein-linked monooxygenase MGC133529 Microsomal monooxygenase OTTHUMP00000020813 OTTHUMP00000046571 P450 ALC P450-J P450C2E P450J P450RLM6 Xenobiotic monooxygenase
Images
ET1703-48_1.jpg Fig1: Western blot analysis of CYP2E1 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1703-48_2.jpg Fig2: Western blot analysis of CYP2E1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
ET1703-48_3.jpg Fig3: ICC staining of CYP2E1 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-48_4.jpg Fig4: ICC staining of CYP2E1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-48_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CYP2E1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-48_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CYP2E1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-48_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-CYP2E1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.