CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95]
cat.: ET1703-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD62P aa 796-830/830. |
| Positive control: | HUVEC cell lysate, human placenta tissue lysate, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P16109 Human |
| Alternative names: | Antigen CD62 CD62 CD62 antigen-like family member P CD62P GMP 140 GMP-140 GMP140 GMRP Granule membrane protein 140 Granulocyte membrane protein GRMP LECAM 3 LECAM3 Leukocyte endothelial cell adhesion molecule 3 Leukocyte-endothelial cell adhesion molecule 3 LYAM3_HUMAN P Selectin P-selectin PADGEM Platelet activation dependent granule-external membrane protein Platelet alpha granule membrane protein PSEL Selectin P (granule membrane protein 140kDa, antigen CD62) SELP |
Images
|
Fig1:
Western blot analysis of CD62P on HUVEC cell lysate with Rabbit anti-CD62P antibody (ET1703-49) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 5 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1703-49, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 90.8 kDa Observed band size: 100 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.