CD62P Recombinant Rabbit Monoclonal Antibody [JM10-95]
cat.: ET1703-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM10-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD62P aa 796-830/830. |
| Positive control: | HUVEC cell lysate, human placenta tissue lysate, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P16109 Human |
| Alternative names: | Antigen CD62 CD62 CD62 antigen-like family member P CD62P GMP 140 GMP-140 GMP140 GMRP Granule membrane protein 140 Granulocyte membrane protein GRMP LECAM 3 LECAM3 Leukocyte endothelial cell adhesion molecule 3 Leukocyte-endothelial cell adhesion molecule 3 LYAM3_HUMAN P Selectin P-selectin PADGEM Platelet activation dependent granule-external membrane protein Platelet alpha granule membrane protein PSEL Selectin P (granule membrane protein 140kDa, antigen CD62) SELP |
Images
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Fig1:
Western blot analysis of CD62P on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysate Lane 2: Human placenta tissue lysate Predicted band size: 91 kDa Observed band size: 100 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.