VAMP2 Recombinant Rabbit Monoclonal Antibody [JM11-00]
cat.: ET1703-50
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC, IP, IHC-P
Clonality: Monoclonal
Clone number: JM11-00
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 13 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human VAMP2 aa 9-42 / 116.
Positive control: Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, U-87 MG, Neuro-2a, C6, SH-SY5Y, mouse brain tissue, rat brain tissue.
Subcellular location: Cell junction, Cell membrane, Cytoplasmic vesicle , Synapse, Synaptosome.
Recommended Dilutions:
  WB
  IF-Cell
  FC
  IP
  IHC-P
1:500-1:2,000
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
1:1,000
Uniprot #: SwissProt: P63027 Human | P63044 Mouse | P63045 Rat
Alternative names: FLJ11460 RATVAMPB RATVAMPIR SYB SYB2 Synaptobrevin 2 Synaptobrevin-2 VAMP 2 VAMP-2 Vamp2 VAMP2_HUMAN Vesicle associated membrane protein 2 Vesicle-associated membrane protein 2 (synaptobrevin 2) Vesicle-associated membrane protein 2
Images
ET1703-50_1.jpg Fig1: Western blot analysis of VAMP2 on different lysates with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 13 kDa
Observed band size: 18 kDa

Exposure time: 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1703-50_2.jpg Fig2: Immunocytochemistry analysis of U-87 MG cells labeling VAMP2 with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1703-50_3.jpg Fig3: Immunocytochemistry analysis of Neuro-2a cells labeling VAMP2 with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1703-50_4.jpg Fig4: Immunocytochemistry analysis of C6 cells labeling VAMP2 with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET1703-50_5.jpg Fig5: Flow cytometric analysis of VAMP2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1703-50_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-50) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-50_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-50) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.