L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05]
cat.: ET1703-51
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM11-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 140 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human L1CAM aa 900-1135 / 1257. |
| Positive control: | A375 cell lysate, A549 cell lysate, HeLa cell lysate, human brain tissue lysate, human kidney tissue, human appendix tissue, human brain tissue, human colon cancer tissue, human prostate cancer tissue. |
| Subcellular location: | Cell membrane, Cell projection, growth cone, axon, dendrite. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000-1:5,000 1:200-1:1,000 1:200 |
| Uniprot #: | SwissProt: P32004 Human |
| Alternative names: | Antigen identified by monoclonal R1 CAML1 CD171 CD171 antigen HSAS HSAS1 Hyd L1 L1 cell adhesion molecule L1-NCAM L1cam L1CAM_HUMAN MASA MIC5 N CAML1 N-CAM-L1 NCAM-L1 NCAML1 Nerve-growth factor-inducible large external glycoprotein Neural cell adhesion molecule L1 NILE OTTHUMP00000025992 S10 SPG1 |
Images
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Fig1:
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (ET1703-51) at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: A549 cell lysate (low expression) Lane 3: HeLa cell lysate Lane 4: Human brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 140 kDa Observed band size: 250 kDa Exposure time: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-51) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-L1CAM antibody (ET1703-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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