ATP5A1 Recombinant Rabbit Monoclonal Antibody [JM110-04]
cat.: ET1703-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JM110-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 60 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ATP5A1 aa 191-234 / 553.
Positive control: SKOV-3 cell lysate, mouse heart tissue lysate, MCF-7 cell lysate, MCF-7, HepG2, Hela, human liver tissue, mouse liver tissue, mouse heart tissue.
Subcellular location: Cell membrane, CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500-1:2,000
1:100-1:500
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P25705 Human | Q03265 Mouse | P15999 Rat
Alternative names: ATP synthase alpha chain, mitochondrial ATP synthase subunit alpha ATP synthase subunit alpha mitochondrial ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 ATP sythase (F1 ATPase) alpha subunit ATP5A Atp5a1 ATP5AL2 ATPA_HUMAN ATPM Epididymis secretory sperm binding protein Li 123m hATP1 HEL-S-123m MC5DN4 mitochondrial Mitochondrial ATP synthetase Mitochondrial ATP synthetase oligomycin resistant Modifier of Min 2 mouse homolog Modifier of Min 2, mouse, homolog of MOM2 OMR ORM OTTHUMP00000163475
Images
ET1703-53_1.jpg Fig1: Western blot analysis of ATP5A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKOV-3 cell lysate
Lane 2: mouse heart tissue lysate
Lane 3: MCF-7 cell lysate
ET1703-53_2.jpg Fig2: ICC staining of ATP5A1 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-53_3.jpg Fig3: ICC staining of ATP5A1 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-53_4.jpg Fig4: ICC staining of ATP5A1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-53_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-53_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-53_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATP5A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-53_8.jpg Fig8: Flow cytometric analysis of ATP5A1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.