CaMKII alpha Recombinant Rabbit Monoclonal Antibody [JM11-07]
cat.: ET1703-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, FC, IHC-P, IF-Tissue, IF-Cell
Clonality: Monoclonal
Clone number: JM11-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CaMKII alpha aa 37-71 / 478.
Positive control: Rat brain tissue lysates, rat testis tissue, mouse brain tissue, SH-SY5Y, Neuro-2a.
Subcellular location: Cell junction, Cell projection, Synapse.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP
  IF-Tissue
  IF-Cell
1:1,000-1:2,000
1:50-1:200
1:50-1:100
1:10-1:50
1:50
1:100
Uniprot #: SwissProt: Q9UQM7 Human | P11798 Mouse | P11275 Rat
Alternative names: Alpha CaMKII Calcium calmodulin dependent protein kinase II Calcium/calmodulin dependent protein kinase II alpha B subunit Calcium/calmodulin dependent protein kinase type II alpha chain Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha Calcium/calmodulin-dependent protein kinase II alpha Calcium/calmodulin-dependent protein kinase II-alpha Calcium/calmodulin-dependent protein kinase type II subunit alpha Calcium/calmodulin-dependent protein kinase type IIA CaM kinase II alpha chain CaM kinase II alpha subunit CaM kinase II subunit alpha CaMK II alpha subunit CaMK-II subunit alpha Camk2a CAMKA CaMKII CaMKIINalpha EC 2.7.11.17 KCC2A_HUMAN KIAA0968 MGC123320 MGC139375 MGC155201 mKIAA0968 PK2CDD PKCCD R74975 zgc:112538 zgc:123320
Images
ET1703-54_1.jpg Fig1: Western blot analysis of CaMKII alpha on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1703-54_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-54_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-54_4.jpg Fig4: Flow cytometric analysis of CaMKII alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1703-54_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling CaMKII alpha with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-54, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1703-54_6.jpg Fig6: Immunocytochemistry analysis of Neuro-2a cells labeling CaMKII alpha with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.