CaMKII alpha Recombinant Rabbit Monoclonal Antibody [JM11-07]
cat.: ET1703-54
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JM11-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CaMKII alpha aa 37-71 / 478. |
| Positive control: | Mouse brain tissue, rat brain tissue lysates, rat testis tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a. |
| Subcellular location: | Cell junction, Cell projection, Synapse. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr IF-Cell FC IP |
1:1,000-1:2,000 1:200-1:500 1:500-1:1,000 1:200-1:500 1:100-1:500 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q9UQM7 Human | P11798 Mouse | P11275 Rat |
| Alternative names: | Alpha CaMKII Calcium calmodulin dependent protein kinase II Calcium/calmodulin dependent protein kinase II alpha B subunit Calcium/calmodulin dependent protein kinase type II alpha chain Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha Calcium/calmodulin-dependent protein kinase II alpha Calcium/calmodulin-dependent protein kinase II-alpha Calcium/calmodulin-dependent protein kinase type II subunit alpha Calcium/calmodulin-dependent protein kinase type IIA CaM kinase II alpha chain CaM kinase II alpha subunit CaM kinase II subunit alpha CaMK II alpha subunit CaMK-II subunit alpha Camk2a CAMKA CaMKII CaMKIINalpha EC 2.7.11.17 KCC2A_HUMAN KIAA0968 MGC123320 MGC139375 MGC155201 mKIAA0968 PK2CDD PKCCD R74975 zgc:112538 zgc:123320 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1: 200 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig2:
Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1: 500 |
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Fig3:
Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1: 500 |
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Fig4: Western blot analysis of CaMKII alpha on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunocytochemistry analysis of mouse primary neural/glia cells labeling CaMKII alpha with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig9:
Immunocytochemistry analysis of Neuro-2a cells labeling CaMKII alpha with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CaMKII alpha antibody (ET1703-54) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.