FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09]
cat.: ET1703-55
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JM11-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 12 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human FKBP12 aa 76-108 / 108.
Positive control: SH-SY5Y cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Cytoplasm, Membrane, Sarcoplasmic reticulum.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P62942 Human | P26883 Mouse | Q62658 Rat
Alternative names: 12 kDa FK506-binding protein 12 kDa FKBP Calstabin 1 FK506 binding protein 1 FK506 binding protein 12 FK506 binding protein 1A (12kD) FK506 binding protein 1A 12kDa FK506 binding protein 1A FK506 binding protein T cell 12 kD FK506 binding protein, T cell, 12 kD FK506 binding protein12 FK506-binding protein 1A FKB1A_HUMAN FKBP 12 FKBP 1A FKBP-12 FKBP-1A FKBP1 FKBP12 FKBP12 Exip3 FKBP12C fkbp1a Immunophilin FKBP12 Peptidyl prolyl cis trans isomerase Peptidyl-prolyl cis-trans isomerase FKBP1A PKC12 PKCI2 PPIase PPIase FKBP1A Protein kinase C inhibitor 2 Rotamase
Images
ET1703-55_1.jpg Fig1: Western blot analysis of FKBP12 on different lysates with Rabbit anti-FKBP12 antibody (ET1703-55) at 1/1,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 12 kDa
Observed band size: 14 kDa

Exposure time: 46 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.