FKBP12 Recombinant Rabbit Monoclonal Antibody [JM11-09]
cat.: ET1703-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM11-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FKBP12 aa 76-108 / 108. |
| Positive control: | SH-SY5Y cell lysate, U937 cell lysate, mouse brain tissue lysates, A431, HepG2, Hela. |
| Subcellular location: | Cytoplasm, Membrane, Sarcoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500-1:1,000 1:100-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: P62942 Human | P26883 Mouse | Q62658 Rat |
| Alternative names: | 12 kDa FK506-binding protein 12 kDa FKBP Calstabin 1 FK506 binding protein 1 FK506 binding protein 12 FK506 binding protein 1A (12kD) FK506 binding protein 1A 12kDa FK506 binding protein 1A FK506 binding protein T cell 12 kD FK506 binding protein, T cell, 12 kD FK506 binding protein12 FK506-binding protein 1A FKB1A_HUMAN FKBP 12 FKBP 1A FKBP-12 FKBP-1A FKBP1 FKBP12 FKBP12 Exip3 FKBP12C fkbp1a Immunophilin FKBP12 Peptidyl prolyl cis trans isomerase Peptidyl-prolyl cis-trans isomerase FKBP1A PKC12 PKCI2 PPIase PPIase FKBP1A Protein kinase C inhibitor 2 Rotamase |
Images
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Fig1:
Western blot analysis of FKBP12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: U937 cell lysate |
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Fig2: Western blot analysis of FKBP12 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of FKBP12 in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of FKBP12 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of FKBP12 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.