Claudin 5 Recombinant Rabbit Monoclonal Antibody [JM11-22]
cat.: ET1703-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JM11-22
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Claudin 5 aa 191-214 / 218.
Positive control: Human brain tissue lysate, human lung tissue lysate, human kidney tissue lysate, human tonsil tissue, human kidney tissue, human lung tissue, human uterus tissue, human lymph nodes tissue
Subcellular location: Cell junction. Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:5,000
1:50-1:200
Uniprot #: SwissProt: O00501 Human
Alternative names: Androgen withdrawal and apoptosis induced protein RVP1 like AWAL BEC 1 BEC1 Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) Claudin-5 Claudin5 CLD5_HUMAN CLDN 5 Cldn5 CPETR L1 CPETRL 1 CPETRL1 TMDVCF TMVCF Transmembrane protein deleted in VCFS Transmembrane protein deleted in velocardiofacial syndrome
Images
ET1703-58_1.jpg Fig1: Western blot analysis of Claudin 5 on different lysates with Rabbit anti-Claudin 5 antibody (ET1703-58) at 1/5,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Human lung tissue lysate
Lane 3: Human kidney tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 20 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1703-58_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Claudin 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-58_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Claudin 5 antibody (ET1703-58) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-58_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Claudin 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-58_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Claudin 5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-58, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-58_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Claudin 5 antibody (ET1703-58) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-58) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.