TIA1 Recombinant Rabbit Monoclonal Antibody [JM42-11]
cat.: ET1703-59
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: JM42-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human TIA1 aa 353-386 / 386.
Positive control: Jurkat cell lysate, K-562 cell lysate, HepG2cell lysate, 293T, A431, Hela, human tonsil tissue, human spleen tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P31483 Human | P52912 Mouse
Alternative names: Cytotoxic granule associated RNA binding protein 1 Cytotoxic granule associated RNA binding protein mTIA-1 Nucleolysin TIA 1 isoform p40 Nucleolysin TIA-1 isoform p40 Nucleolysin TIA1 isoform p40 p40 TIA 1 p40-TIA-1 (containing p15-TIA-1) p40-TIA-1 RNA binding protein TIA 1 RNA binding protein TIA1 RNA-binding protein TIA-1 T-cell-restricted intracellular antigen-1 TIA 1 TIA 1 cytotoxic granule associated RNA binding protein Tia TIA-1 TIA1 TIA1 cytotoxic granule associated RNA binding protein TIA1 cytotoxic granule associated RNA binding protein like 1 TIA1 protein TIA1_HUMAN TIAL1 TIAR WDM
Images
ET1703-59_1.jpg Fig1: Western blot analysis of TIA1 on different lysates with Rabbit anti-TIA1 antibody (ET1703-59) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: HepG2cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 41/43 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-59) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1703-59_2.jpg Fig2: ICC staining of TIA1 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-59, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-59_3.jpg Fig3: ICC staining of TIA1 in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-59, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-59_4.jpg Fig4: ICC staining of TIA1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-59, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-59_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TIA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-59_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TIA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.