ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11]
cat.: ET1703-60
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IHC-P
Clonality: Monoclonal
Clone number: JM32-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 84 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ADAM10 aa 715-748 / 748.
Positive control: Jurkat cell lysate, HeLa cell lysate, human lung tissue lysate, RAW264.7 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat spleen tissue lysate, human thyroid carcinoma tissue, human stomach tissue.
Subcellular location: Cell membrane. Endomembrane system.
Recommended Dilutions:
  WB
  IP
  IHC-P
1:500-1:2,000
1:10-1:50
1:100
Uniprot #: SwissProt: O14672 Human | O35598 Mouse | Q10743 Rat
Alternative names: A disintegrin and metalloprotease domain 10 A disintegrin and metalloproteinase domain 10 AD 10 AD10 AD18 ADA10_HUMAN ADAM 10 ADAM metallopeptidase domain 10 ADAM10 CD 156c CD156c CD156c antigen CDw156 disintegrin and metalloproteinase domain containing protein 10 Disintegrin and metalloproteinase domain-containing protein 10 HsT 18717 HsT18717 Kuz Kuzbanian Kuzbanian protein homolog Kuzbanian, Drosophila, homolog of MADM Mammalian disintegrin metalloprotease Mammalian disintegrin-metalloprotease RAK
Images
ET1703-60_1.jpg Fig1: Western blot analysis of ADAM10 on different lysates with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: Human lung tissue lysate (30 µg/Lane)
Lane 4: RAW264.7 cell lysate (15 µg/Lane)
Lane 5: Mouse placenta tissue lysate (30 µg/Lane)
Lane 6: Mouse brain tissue lysate (30 µg/Lane)
Lane 7: Rat brain tissue lysate (30 µg/Lane)
Lane 8: Rat spleen tissue lysate (30 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 84 kDa
Observed band size: 75/100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1703-60_2.jpg Fig2: All lanes: Western blot analysis of ADAM10 with anti-ADAM10 antibody [SP08-04] (ET1703-60) at 1/1,000 dilution.

Lane 1: Wild-type A549 whole cell lysate.
Lane 2: ADAM10 knockout A549 whole cell lysate.

ET1703-60 was shown to specifically react with ADAM10 in wild-type A549 cells. No band was observed when ADAM10 knockout sample was tested. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-ADAM10 antibody (ET1703-60, 1/1,000) and Anti-Vinculin antibody (ET1705-94, 1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
ET1703-60_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-60) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-60_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ADAM10 antibody (ET1703-60) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-60) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.