Podoplanin Recombinant Rabbit Monoclonal Antibody [JM31-21]
cat.: ET1703-61
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JM31-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Podoplanin aa 35-75 / 162.
Positive control: Human lung tissue lysates, human lung tissue, human breast tissue.
Subcellular location: Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:400
Uniprot #: SwissProt: Q86YL7 Human
Alternative names: Aggrus Glycoprotein 36 KD Glycoprotein 36 gp 36 GP 38 GP 40 gp36 GP38 GP40 HT1A 1 HT1A1 hT1alpha 1 hT1alpha 2 hT1alpha1 hT1alpha2 Lung type I cell membrane associated glycoprotein Lung type I cell membrane associated glycoprotein isoform a Lung type I cell membrane associated glycoprotein T1A 2 OTS 8 OTS8 OTTHUMP00000009640 OTTHUMP00000044504 PA2.26 PA2.26 antigen Pdpn PDPN_HUMAN Podoplanin PSEC0003 PSEC0025 T1 alpha T1 ALPHA GENE T1-alpha T1A 2 T1A TI1A TIA 2 TIA2
Images
ET1703-61_1.jpg Fig1: Western blot analysis of Podoplanin on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1703-61_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Podoplanin antibody (ET1703-61) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-61) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-61_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Podoplanin antibody (ET1703-61) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-61) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.