Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IP, IF-Cell, IF-Tissue |
Clonality: | Monoclonal |
Clone number: | JM11-13 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 25 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Peroxiredoxin 6 aa 175-224 / 224. |
Positive control: | HeLa cell lysate, K-562 cell lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate. |
Subcellular location: | Cytoplasm. Lysosome. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IP |
1:1,000 1:50-1:200 1:50-1:200 1:10-1:50 |
Uniprot #: | SwissProt: P30041 Human | O08709 Mouse | O35244 Rat |
Alternative names: | 1 Cys 1 Cys peroxiredoxin 1 Cys PRX 1 cysPrx 1-Cys peroxiredoxin 1-Cys PRX 24 kDa protein 9430088D19Rik AA690119 Acidic calcium independent phospholipase A2 Acidic calcium-independent phospholipase A2 aiPLA2 Antioxidant protein 2 AOP2 Aop2 rs3 Brp 12 Ciliary body glutathione peroxidase CP 3 EC 1.11.1.15 EC 1.11.1.7 EC 3.1.1. Epididymis secretory sperm binding protein Li 128m GPx HEL S 128m KIAA0106 Liver 2D page spot 40 Ltw4 Lvtw 4 MGC46173 mKIAA0106 Non selenium glutathione peroxidase Non-selenium glutathione peroxidase NSGPx ORF06 OTTHUMP00000032693 p29 Peroxiredoxin-6 Peroxiredoxin6 PHGPx Phospholipase A2 lysosomal PLA2 PRDX 6 Prdx5 PRDX6 Prdx6 rs3 PRDX6_HUMAN PRX Red blood cells page spot 12 Thiol specific antioxidant protein |
Fig1:
Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (ET1703-62) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |