Peroxiredoxin 6 Recombinant Rabbit Monoclonal Antibody [JM11-13]
cat.: ET1703-62
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JM11-13
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Peroxiredoxin 6 aa 175-224 / 224.
Positive control: HeLa cell lysate, K-562 cell lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate.
Subcellular location: Cytoplasm. Lysosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IP

1:1,000
1:50-1:200
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: P30041 Human | O08709 Mouse | O35244 Rat
Alternative names: 1 Cys 1 Cys peroxiredoxin 1 Cys PRX 1 cysPrx 1-Cys peroxiredoxin 1-Cys PRX 24 kDa protein 9430088D19Rik AA690119 Acidic calcium independent phospholipase A2 Acidic calcium-independent phospholipase A2 aiPLA2 Antioxidant protein 2 AOP2 Aop2 rs3 Brp 12 Ciliary body glutathione peroxidase CP 3 EC 1.11.1.15 EC 1.11.1.7 EC 3.1.1. Epididymis secretory sperm binding protein Li 128m GPx HEL S 128m KIAA0106 Liver 2D page spot 40 Ltw4 Lvtw 4 MGC46173 mKIAA0106 Non selenium glutathione peroxidase Non-selenium glutathione peroxidase NSGPx ORF06 OTTHUMP00000032693 p29 Peroxiredoxin-6 Peroxiredoxin6 PHGPx Phospholipase A2 lysosomal PLA2 PRDX 6 Prdx5 PRDX6 Prdx6 rs3 PRDX6_HUMAN PRX Red blood cells page spot 12 Thiol specific antioxidant protein
Images
ET1703-62_1.jpg Fig1: Western blot analysis of Peroxiredoxin 6 on different lysates with Rabbit anti-Peroxiredoxin 6 antibody (ET1703-62) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Rat lung tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 25 kDa
Observed band size: 27 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.