ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]
cat.: ET1703-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM11-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ERp57 aa 175-208 / 505. |
| Positive control: | HepG2 cell lysate, LNCaP cell lysate, human kidney tissue, human pancreas tissue, human uterus tissue. |
| Subcellular location: | Endoplasmic reticulum lumen. Melanosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P30101 Human |
| Alternative names: | 58 kDa glucose regulated protein 58 kDa glucose-regulated protein 58 kDa microsomal protein Disulfide isomerase ER 60 Disulfide isomerase ER-60 Endoplasmic reticulum resident protein 57 Endoplasmic reticulum resident protein 60 ER p57 ER protein 57 ER protein 60 ERp 57 ERp57 ERp60 ERp61 Glucose Regulated Protein 58 Kd GRP 57 GRP 58 GRP57 HsT17083 p58 PDIA 3 PDIA3 PDIA3_HUMAN Phospholipase C alpha PI PLC Protein disulfide isomerase A3 Protein disulfide isomerase family A member 3 Protein disulfide-isomerase A3 |
Images
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Fig1:
Western blot analysis of ERp57 on different lysates with Rabbit anti-ERp57 antibody (ET1703-68) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LNCaP cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.