GAD67 Recombinant Rabbit Monoclonal Antibody [JM11-11]
cat.: ET1703-71
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JM11-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GAD67 aa 60-99 / 594. |
| Positive control: | Hela cells lysates, SH-SY5Y cells lysates, human cerebellum tissue, mouse hippocampus tissue, mouse cerebral cortex tissue. |
| Subcellular location: | Cytoplasm, Plasma Membrane. |
| Recommended Dilutions:
WB IP IHC-P IHC-Fr |
1:500-1:1,000 1:50-1:100 1:200 1:50 |
| Uniprot #: | SwissProt: Q99259 Human | P48318 Mouse | P18088 Rat |
| Alternative names: | 67 kDa glutamic acid decarboxylase CPSQ1 DCE1 DCE1_HUMAN EC 4.1.1.15 FLJ45882 GAD 67 GAD GAD-67 GAD1 Glutamate decarboxylase 1 (brain, 67kDa) Glutamate decarboxylase 1 Glutamate decarboxylase 1 brain 67kD Glutamate decarboxylase 1 brain 67kDa Glutamate decarboxylase 67 kDa isoform Glutamate decarboxylase, brain, 67-KD OTTHUMP00000041055 SCP |
Images
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Fig1: Western blot analysis of GAD67 on Hela cells lysates using anti-GAD67 antibody at 1/500 dilution. |
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Fig2: Western blot analysis of GAD67 on SH-SY5Y cells lysates using anti-GAD67 antibody at 1/500 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GAD67 antibody (ET1703-71) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-71) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling GAD67 with Rabbit anti-GAD67 antibody (ET1703-71). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-71, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig5:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling GAD67 with Rabbit anti-GAD67 antibody (ET1703-71). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-71, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.