GAD67 Recombinant Rabbit Monoclonal Antibody [JM11-11]
cat.: ET1703-71
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM11-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GAD67 aa 60-99 / 594. |
| Positive control: | HeLa cell lysate, SH-SY5Y cell lysate. |
| Subcellular location: | Cytoplasm, Plasma Membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:500-1:1,000 1:50-1:100 1:200 |
| Uniprot #: | SwissProt: Q99259 Human | P48318 Mouse | P18088 Rat |
| Alternative names: | 67 kDa glutamic acid decarboxylase CPSQ1 DCE1 DCE1_HUMAN EC 4.1.1.15 FLJ45882 GAD 67 GAD GAD-67 GAD1 Glutamate decarboxylase 1 (brain, 67kDa) Glutamate decarboxylase 1 Glutamate decarboxylase 1 brain 67kD Glutamate decarboxylase 1 brain 67kDa Glutamate decarboxylase 67 kDa isoform Glutamate decarboxylase, brain, 67-KD OTTHUMP00000041055 SCP |
Images
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Fig1:
Western blot analysis of GAD67 on different lysates with Rabbit anti-GAD67 antibody (ET1703-71) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.