NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10]
cat.: ET1703-73
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: JM11-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human NeuroD1 aa 1-44 / 356.
Positive control: Human brain tissue lysate, SH-SY5Y cell lysate, Li-7 cell lysates.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IP

1:500-1:1,000
1:10-1:50
Uniprot #: SwissProt: Q13562 Human | Q60867 Mouse | Q64289 Rat
Alternative names: atonal basic helix loop helix transcription factor BETA 2 Beta cell E box transactivator 2 BETA2 BHF 1 BHF1 bHLHa3 class A basic helix loop helix protein 3 Class A basic helix-loop-helix protein 3 MODY 6 MODY6 NDF1_HUMAN NeuroD NeuroD1 Neurogenic differentiation 1 Neurogenic differentiation factor 1 neurogenic helix loop helix protein NEUROD Neuronal differentiation 1
Images
ET1703-73_1.jpg Fig1: Western blot analysis of NeuroD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: SH-SY5Y cell lysate
ET1703-73_2.jpg Fig2: Western blot analysis of NeuroD1 on Li-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.