NeuroD1 Recombinant Rabbit Monoclonal Antibody [JM11-10]
cat.: ET1703-73
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, IHC-P
Clonality: Monoclonal
Clone number: JM11-10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human NeuroD1 aa 1-44 / 356.
Positive control: Human brain tissue lysate, SH-SY5Y cell lysate, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IP
  IHC-P

1:500-1:1,000
1:10-1:50
1:200-1:500
Uniprot #: SwissProt: Q13562 Human | Q60867 Mouse | Q64289 Rat
Alternative names: atonal basic helix loop helix transcription factor BETA 2 Beta cell E box transactivator 2 BETA2 BHF 1 BHF1 bHLHa3 class A basic helix loop helix protein 3 Class A basic helix-loop-helix protein 3 MODY 6 MODY6 NDF1_HUMAN NeuroD NeuroD1 Neurogenic differentiation 1 Neurogenic differentiation factor 1 neurogenic helix loop helix protein NEUROD Neuronal differentiation 1
Images
ET1703-73_1.jpg Fig1: Western blot analysis of NeuroD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: SH-SY5Y cell lysate
ET1703-73_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-73_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-73_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NeuroD1 antibody (ET1703-73) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.