NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26]
cat.: ET1703-75
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IF-Tissue, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JM11-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NMDAR1 aa 870-910. |
| Positive control: | MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, mouse cerebral cortex tissue, rat cerebral cortex tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, rat cerebral cortex tissue. |
| Subcellular location: | Cell membrane, postsynaptic cell membrane, postsynaptic density. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IHC-Fr |
1:1,000-1:5,000 1:200-1:500 1:1,000 1:500 |
| Uniprot #: | SwissProt: Q05586 Human | P35438 Mouse | P35439 Rat |
| Alternative names: | GluN1 Glutamate [NMDA] receptor subunit zeta-1 Glutamate receptor ionotropic N methyl D aspartate 1 Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 glutamate receptor ionotropic, NMDA 1 Grin1 MRD8 N methyl D aspartate receptor N methyl D aspartate receptor channel subunit zeta 1 N methyl D aspartate receptor subunit NR1 N-methyl-D-aspartate receptor subunit NR1 NMD-R1 NMDA 1 NMDA R1 NMDA receptor 1 NMDA1 NMDAR NMDZ1_HUMAN NR1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Western blot analysis of NMDAR1 on different lysates with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane) Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane) Predicted band size: 105 kDa Observed band size: 120 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-75) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig7:
Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.