Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JM10-64 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 48 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human FOXA2 aa 1-50/457. |
Positive control: | HT-29 cell lysates. |
Subcellular location: | Nucleus. Cytoplasm. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q9Y261 Human |
Alternative names: | Forkhead box A2 Forkhead box protein A2 FOX A2 foxa2 FOXA2_HUMAN Hepatic nuclear factor 3 beta Hepatocyte nuclear factor 3 Hepatocyte nuclear factor 3 beta Hepatocyte nuclear factor 3-beta HNF 3B HNF-3-beta HNF-3B HNF3B MGC19807 TCF 3B TCF-3B TCF3B Transcription factor 3B |
Fig1: Western blot analysis of FOXA2 on HT-29 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |