Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IP |
Clonality: | Monoclonal |
Clone number: | JM10-60 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 92 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human FGFR2 aa 30-70 / 821. |
Positive control: | MCF-7 cell lysate, Jurkat cell lysate. |
Subcellular location: | Cell membrane, Golgi apparatus, Cytoplasmic vesicle. |
Recommended Dilutions:
WB IP |
1:1,000-1:2,000 1:10-1:50 |
Uniprot #: | SwissProt: P21802 Human | P21803 Mouse Entrez Gene: 25022 Rat |
Alternative names: | bacteria-expressed kinase BBDS BEK BEK fibroblast growth factor receptor BFR1 CD332 CD332 antigen CEK3 CFD1 Craniofacial dysostosis 1 ECT1 FGF receptor FGFR 2 FGFR-2 Fgfr2 FGFR2_HUMAN Fibroblast growth factor receptor 2 Hydroxyaryl protein kinase Jackson Weiss syndrome JWS K SAM K-sam Keratinocyte growth factor receptor 2 Keratinocyte growth factor receptor KGFR KSAM protein tyrosine kinase, receptor like 14 soluble FGFR4 variant 4 TK14 TK25 |
Fig1:
Western blot analysis of FGFR2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate |