FGFR2 Recombinant Rabbit Monoclonal Antibody [JM10-60]
cat.: ET1703-78
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM10-60 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FGFR2 aa 30-70 / 821. |
| Positive control: | HT-29 cell lysate, HaCaT cell lysate, PC-12 cell lysate, L6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, MCF7 cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cell membrane, Golgi apparatus, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IP |
1:1,000-1:2,000 1:10-1:50 |
| Uniprot #: | SwissProt: P21802 Human | P21803 Mouse Entrez Gene: 25022 Rat |
| Alternative names: | bacteria-expressed kinase BBDS BEK BEK fibroblast growth factor receptor BFR1 CD332 CD332 antigen CEK3 CFD1 Craniofacial dysostosis 1 ECT1 FGF receptor FGFR 2 FGFR-2 Fgfr2 FGFR2_HUMAN Fibroblast growth factor receptor 2 Hydroxyaryl protein kinase Jackson Weiss syndrome JWS K SAM K-sam Keratinocyte growth factor receptor 2 Keratinocyte growth factor receptor KGFR KSAM protein tyrosine kinase, receptor like 14 soluble FGFR4 variant 4 TK14 TK25 |
Images
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Fig1:
Western blot analysis of FGFR2 on different lysates with Rabbit anti-FGFR2 antibody (ET1703-78) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: HaCaT cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: L6 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 145 kDa Exposure time: Lane 1-4: 1 minute 18 seconds; Lane 5-6: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-78) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of FGFR2 on different lysates with Rabbit anti-FGFR2 antibody (ET1703-78) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 92 kDa Observed band size: 145 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-78) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.