Endothelin B Receptor Recombinant Rabbit Monoclonal Antibody [JM74-10]
cat.: ET1703-79
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Zebrafish
Applications: WB, IP
Clonality: Monoclonal
Clone number: JM74-10
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Endothelin B Receptor aa 58-91 / 442.
Positive control: Raji cell lysates.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IP

1:2,000
1:10-1:50
Uniprot #: SwissProt: P24530 Human
Alternative names: ABCDS Ednra EDNRB EDNRB_HUMAN Endothelin B receptor Endothelin B receptor precursor Endothelin receptor Non selective type Endothelin receptor non-selective type Endothelin receptor type B ET B ET-B ET-BR ETB ETBR ETRB Hirschsprung disease 2 HSCR HSCR2 OTTHUMP00000018534 OTTHUMP00000178736 WS4A
Images
ET1703-79_1.jpg Fig1: Western blot analysis of Endothelin B Receptor on Raji cell lysate with Rabbit anti-Endothelin B Receptor antibody (ET1703-79) at 1/2,000 dilution.

Lysates/proteins at 20 µg/Lane.
Exposure time: 17 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1703-79, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 50 kDa
Observed band size: 55 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.