| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Zebrafish |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JM74-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Endothelin B Receptor aa 58-91 / 442. |
| Positive control: | Raji cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IP |
1:2,000 1:10-1:50 |
| Uniprot #: | SwissProt: P24530 Human |
| Alternative names: | ABCDS Ednra EDNRB EDNRB_HUMAN Endothelin B receptor Endothelin B receptor precursor Endothelin receptor Non selective type Endothelin receptor non-selective type Endothelin receptor type B ET B ET-B ET-BR ETB ETBR ETRB Hirschsprung disease 2 HSCR HSCR2 OTTHUMP00000018534 OTTHUMP00000178736 WS4A |
|
Fig1:
Western blot analysis of Endothelin B Receptor on Raji cell lysate with Rabbit anti-Endothelin B Receptor antibody (ET1703-79) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 17 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1703-79, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 55 kDa |