Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Zebrafish |
Applications: | WB, IP |
Clonality: | Monoclonal |
Clone number: | JM74-10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 50 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Endothelin B Receptor aa 58-91 / 442. |
Positive control: | Zebrafish tissue lysate, Raji cell lysate, JAR cell lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB IP |
1:500-1:2,000 1:10-1:50 |
Uniprot #: | SwissProt: P24530 Human |
Alternative names: | ABCDS Ednra EDNRB EDNRB_HUMAN Endothelin B receptor Endothelin B receptor precursor Endothelin receptor Non selective type Endothelin receptor non-selective type Endothelin receptor type B ET B ET-B ET-BR ETB ETBR ETRB Hirschsprung disease 2 HSCR HSCR2 OTTHUMP00000018534 OTTHUMP00000178736 WS4A |
Fig1:
Western blot analysis of Endothelin B Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Zebrafish tissue lysate Lane 2: Raji cell lysate Lane 3: JAR cell lysate |