Rac1-2-3 Recombinant Rabbit Monoclonal Antibody [JM11-29]
cat.: ET1703-80
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JM11-29
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of Human RAC1 aa 1-192 / 192.
Positive control: MCF-7 cell lysate, PC-12 cell lysate, 293T, Hela, NIH/3T3, human spleen tissue, mouse brain tissue, rat brain tissue.
Subcellular location: Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:2,000
1:50-1:200
1:200
1:200
Uniprot #: SwissProt: P63000 Human | P15153 Human | P60763 Human | P63001 Mouse | Q6RUV5 Rat
Alternative names: Cell migration inducing gene 5 protein EN 7 OTTMUSP00000004488 p21 Rac3 p21-Rac3 Rac1B RAC3 RAC3_HUMAN RAS related C3 botulinum substrate 3 Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) Ras-related C3 botulinum toxin substrate 3 Rho family small GTP binding protein Rac3 RP23-84C12.18 EN-7 EN7 GX HSPC 022 HSPC022 p21 Rac 2 p21 Rac2 p21-Rac2 p21Rac2 RAC 2 Rac2 RAC2_HUMAN Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 Ras related C3 botulinum toxin substrate 2 Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) Ras related C3 botulinum toxin substrate 3 Ras-related C3 botulinum toxin substrate 2 Rho family small GTP binding protein Rac 2 Rho family small GTP binding protein Rac2 Small G protein Cell migration-inducing ge......
Images
ET1703-80_1.jpg Fig1: Western blot analysis of Rac1-2-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: PC-12 cell lysate
ET1703-80_2.jpg Fig2: ICC staining of Rac1-2-3 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-80_3.jpg Fig3: ICC staining of Rac1-2-3 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-80_4.jpg Fig4: ICC staining of Rac1-2-3 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1703-80_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Rac1-2-3 antibody (ET1703-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-80_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Rac1-2-3 antibody (ET1703-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-80_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Rac1-2-3 antibody (ET1703-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1703-80_8.jpg Fig8: Immunofluorescence analysis of paraffin-embedded mouse embryonic brain tissue labeling Rac1-2-3 with Rabbit anti-Rac1-2-3 antibody (ET1703-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-80, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1703-80_9.jpg Fig9: Immunofluorescence analysis of paraffin-embedded mouse embryonic eye tissue labeling Rac1-2-3 with Rabbit anti-Rac1-2-3 antibody (ET1703-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-80, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.