RPE65 Recombinant Rabbit Monoclonal Antibody [JM61-51]
cat.: ET1703-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM61-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPE65 aa 441-484 / 533. |
| Positive control: | Mouse eyeball tissue lysate, Rat eyeball tissue lysate, rat eyeball tissue, mouse eyeball tissue. |
| Subcellular location: | Cytoplasm. Cell membrane. |
| Recommended Dilutions:
WB IP IHC-P IF-Tissue |
1:2,000 1:10-1:50 1:1,000 1:500 |
| Uniprot #: | SwissProt: Q16518 Human | Q91ZQ5 Mouse | O70276 Rat |
| Alternative names: | All-trans-retinyl-palmitate hydrolase LCA 2 LCA2 Leber congenital amaurosis mRPE 65 mRPE65 p63 rd 12 rd12 Retinal pigment epithelium specific 61 kDa protein Retinal pigment epithelium specific 65 kDa protein Retinal pigment epithelium specific protein Retinal pigment epithelium specific protein 65kDa Retinal pigment epithelium-specific 65 kDa protein Retinitis pigmentosa 20 Retinoid isomerohydrolase Retinol isomerase RP 20 RP20 RPE 65 RPE65 RPE65_HUMAN sRPE 65 sRPE65 |
Images
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Fig1:
Western blot analysis of RPE65 on different lysates with Rabbit anti-RPE65 antibody (ET1703-82) at 1/2,000 dilution. Lane 1: Mouse eyeball tissue lysate Lane 2: Rat eyeball tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-RPE65 antibody (ET1703-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-RPE65 antibody (ET1703-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunofluorescence analysis of paraffin-embedded mouse eyeball tissue labeling RPE65 with Rabbit anti-RPE65 antibody (ET1703-82) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-82, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.