RPE65 Recombinant Rabbit Monoclonal Antibody [JM61-51]
cat.: ET1703-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM61-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPE65 aa 441-484 / 533. |
| Positive control: | Mouse eyeball tissue lysates, rat eyeball tissue, mouse eyeball tissue. |
| Subcellular location: | Cytoplasm. Cell membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:1,000-1:2,000 1:10-1:50 1:1,000 |
| Uniprot #: | SwissProt: Q16518 Human | Q91ZQ5 Mouse | O70276 Rat |
| Alternative names: | All-trans-retinyl-palmitate hydrolase LCA 2 LCA2 Leber congenital amaurosis mRPE 65 mRPE65 p63 rd 12 rd12 Retinal pigment epithelium specific 61 kDa protein Retinal pigment epithelium specific 65 kDa protein Retinal pigment epithelium specific protein Retinal pigment epithelium specific protein 65kDa Retinal pigment epithelium-specific 65 kDa protein Retinitis pigmentosa 20 Retinoid isomerohydrolase Retinol isomerase RP 20 RP20 RPE 65 RPE65 RPE65_HUMAN sRPE 65 sRPE65 |
Images
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Fig1: Western blot analysis of RPE65 on mouse eyeball tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-RPE65 antibody (ET1703-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-RPE65 antibody (ET1703-82) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.