RPE65 Recombinant Rabbit Monoclonal Antibody [JM61-51]
cat.: ET1703-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr, IP |
| Clonality: | Monoclonal |
| Clone number: | JM61-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPE65 aa 441-484 / 533. |
| Positive control: | Mouse eyeball tissue lysate, Rat eyeball tissue lysate, rat eyeball tissue, mouse eyeball tissue. |
| Subcellular location: | Cytoplasm. Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr IP |
1:2,000 1:1,000 1:500 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q16518 Human | Q91ZQ5 Mouse | O70276 Rat |
| Alternative names: | All-trans-retinyl-palmitate hydrolase LCA 2 LCA2 Leber congenital amaurosis mRPE 65 mRPE65 p63 rd 12 rd12 Retinal pigment epithelium specific 61 kDa protein Retinal pigment epithelium specific 65 kDa protein Retinal pigment epithelium specific protein Retinal pigment epithelium specific protein 65kDa Retinal pigment epithelium-specific 65 kDa protein Retinitis pigmentosa 20 Retinoid isomerohydrolase Retinol isomerase RP 20 RP20 RPE 65 RPE65 RPE65_HUMAN sRPE 65 sRPE65 |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Retina Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1703-82, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Retina Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1703-82, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig3:
Western blot analysis of RPE65 on different lysates with Rabbit anti-RPE65 antibody (ET1703-82) at 1/2,000 dilution. Lane 1: Mouse eyeball tissue lysate Lane 2: Rat eyeball tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1703-82, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1703-82, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Retina Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1703-82, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.