mGluR1 Recombinant Rabbit Monoclonal Antibody [JM11-61]
cat.: ET1703-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JM11-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 132 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human mGluR1 aa 280-420 / 1194. |
| Positive control: | Mouse cerebellum tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue, mouse kidney tissue, rat cerebellum tissue, mouse cerebellum tissue, human cerebellum tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr |
1:500-1:1,000 1:50-1:1,000 1:50-1:200 1:200 |
| Uniprot #: | SwissProt: Q13255 Human | P97772 Mouse | P23385 Rat |
| Alternative names: | Glutamate receptor metabotropic 1 glutamate receptor, metabotropic 1 GPRC1A GRM1 GRM1-Alpha GRM1_HUMAN GRM1A Metabotropic glutamate receptor 1 mGlu1 mGluR1 MGLUR1-Alpha MGLUR1A SCAR13 |
Images
|
Fig1:
Western blot analysis of mGluR1 on different lysates with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Rat cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 132 kDa Observed band size: 300 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-84, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.