Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Streptococcus pyogenes |
Applications: | WB, ICC/IF, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JM11-55 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Protein A affinity purified. |
Molecular weight: | 158 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Streptococcus pyogenes cas9 aa 740-980. |
Positive control: | 293 cell lysate transfected with CRISPR-Cas9, 293T cell lysate transfected with CRISPR-Cas9. |
Recommended Dilutions:
WB ICC/IF IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: Q99ZW2 StreptococcusPyogenes |
Alternative names: | Cas9 antibody CRISPR-associated endonuclease Cas9/Csn1 antibody CRISPR-Cas9/Csn1 antibody csn1 antibody SpyCas9 antibody |
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Fig1:
Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysates transfected with CRISPR-Cas9 Lane 2: 293T cell lysates transfected with CRISPR-Cas9 |