CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55]
cat.: ET1703-85
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Streptococcus pyogenes |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JM11-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 158 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Streptococcus pyogenes cas9 aa 730-990 / 1368. |
| Positive control: | 293T transfected with Cas9 cell lysates. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q99ZW2 StreptococcusPyogenes |
| Alternative names: | Cas9 CRISPR-associated endonuclease Cas9/Csn1 CRISPR-Cas9/Csn1 csn1 SpyCas9 |
Images
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Fig1:
Western blot analysis of CRISPR-Cas9 SP on 293T transfected with Cas9 cell lysates with Rabbit anti-CRISPR-Cas9 SP antibody (ET1703-85) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 158 kDa Observed band size: 158 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-85) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.