Anti-CRISPR-Cas9 SP antibody [JM11-55]
cat.: ET1703-85
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Streptococcus pyogenes
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JM11-55
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 158 kDa
Isotype: IgG
Immunogen: Recombinant protein within Streptococcus pyogenes cas9 aa 740-980.
Positive control: 293 cell lysate transfected with CRISPR-Cas9, 293T cell lysate transfected with CRISPR-Cas9.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q99ZW2 StreptococcusPyogenes
Alternative names: Cas9 antibody CRISPR-associated endonuclease Cas9/Csn1 antibody CRISPR-Cas9/Csn1 antibody csn1 antibody SpyCas9 antibody
Images
ET1703-85_1.jpg Fig1: Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysates transfected with CRISPR-Cas9
Lane 2: 293T cell lysates transfected with CRISPR-Cas9
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.