Anti-Phospho-POLR2A (S2) antibody [JM11-51]
cat.: ET1703-86
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JM11-51
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 250 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser2 of human POLR2A.
Positive control: MCF-7, PC-12, Hela, human tonsil tissue, mouse testis tissue, rat brain tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:2,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P24928 Human | P08775 Mouse
Alternative names: DNA directed RNA polymerase II A antibody DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody DNA-directed RNA polymerase II subunit A antibody DNA-directed RNA polymerase II subunit RPB1 antibody DNA-directed RNA polymerase III largest subunit antibody hRPB220 antibody hsRPB1 antibody POLR2 antibody Polr2a antibody POLRA antibody Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody Polymerase (RNA) II (DNA directed) polypeptide A antibody RNA polymerase II subunit B1 antibody RNA-directed RNA polymerase II subunit RPB1 antibody RPB1 antibody RPB1_HUMAN antibody RPBh1 antibody RpIILS antibody RPO2 antibody RPOL2 antibody
Images
ET1703-86_1.jpg Fig1: Western blot analysis of Phospho-POLR2A (S2) on differnt cells lysates using anti- Phospho-POLR2A (S2) antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: MCF-7
ET1703-86_2.jpg Fig2: ICC staining Phospho-POLR2A (S2) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-86_3.jpg Fig3: ICC staining Phospho-POLR2A (S2) in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-86_4.jpg Fig4: ICC staining Phospho-POLR2A (S2) in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1703-86_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.
ET1703-86_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.
ET1703-86_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.
ET1703-86_8.jpg Fig8: Flow cytometric analysis of Hela cells with Phospho-POLR2A (S2) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.