delta Sarcoglycan Recombinant Rabbit Monoclonal Antibody [JM61-10]
cat.: ET1703-88
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM61-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human delta Sarcoglycan aa 221-265 / 289. |
| Positive control: | A549 cell lysate, human stomach carcinoma tissue. |
| Subcellular location: | Cytoskeleton, sarcolemma. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q92629 Human |
| Alternative names: | 35 kDa dystrophin associated glycoprotein 35 kDa dystrophin-associated glycoprotein 35DAG CMD1L DAGD Delta-sarcoglycan Delta-SG Dystrophin associated glycoprotein delta sarcoglycan LGMD2F MGC22567 Placental delta sarcoglycan Sarcoglycan delta (35 kDa dystrophin associated glycoprotein) SG delta SGCD SGCD_HUMAN SGCDP SGD |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-delta Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-88, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of delta Sarcoglycan on A549 cell lysate with Rabbit anti-delta Sarcoglycan antibody (ET1703-88) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1703-88, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 32 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.