delta Sarcoglycan Recombinant Rabbit Monoclonal Antibody [JM61-10]
cat.: ET1703-88
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM61-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human delta Sarcoglycan aa 221-265 / 289. |
| Positive control: | Human lung tissue lysate, human skeletal muscle tissue lysate, human stomach carcinoma tissue. |
| Subcellular location: | Cytoskeleton, sarcolemma. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q92629 Human |
| Alternative names: | 35 kDa dystrophin associated glycoprotein 35 kDa dystrophin-associated glycoprotein 35DAG CMD1L DAGD Delta-sarcoglycan Delta-SG Dystrophin associated glycoprotein delta sarcoglycan LGMD2F MGC22567 Placental delta sarcoglycan Sarcoglycan delta (35 kDa dystrophin associated glycoprotein) SG delta SGCD SGCD_HUMAN SGCDP SGD |
Images
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Fig1:
Western blot analysis of delta Sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human lung tissue lysate Lane 2: Human skeletal muscle tissue lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-delta Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-88, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.