Anti-MIF antibody [JM11-64]
cat.: ET1703-89
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, FC
Clonality: Monoclonal
Clone number: JM11-64
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 12 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: Jurkat, Hela, THP-1, mouse brain.
Subcellular location: Secreted. Cytoplasm.
Recommended Dilutions:
  WB
  IP
  FC

1:500-1:2,000
1:10-1:50
1:50-1:100
Uniprot #: SwissProt: P14174 Human | P34884 Mouse | P30904 Rat
Alternative names: GIF GLIF Glycosylation inhibiting factor Glycosylation-inhibiting factor L-dopachrome isomerase L-dopachrome tautomerase Macrophage migration inhibitory factor (glycosylation-inhibiting factor) Macrophage migration inhibitory factor MIF MIF protein MIF_HUMAN MMIF Phenylpyruvate tautomerase
Images
ET1703-89_1.jpg Fig1: Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.
Positive control:
Line 1: Mouse brain
Line 2: Jurkat
Line 3: Hela
ET1703-89_2.jpg Fig2: Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.