MIF Recombinant Rabbit Monoclonal Antibody [JM11-64]
cat.: ET1703-89
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JM11-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 12 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MIF aa 60-90. |
| Positive control: | Mouse brain tissue lysate, Jurkat cell lysate, Hela cell lysate, THP-1. |
| Subcellular location: | Secreted. Cytoplasm. |
| Recommended Dilutions:
WB IP FC |
1:500-1:2,000 1:10-1:50 1:50-1:100 |
| Uniprot #: | SwissProt: P14174 Human | P34884 Mouse | P30904 Rat |
| Alternative names: | GIF GLIF Glycosylation inhibiting factor Glycosylation-inhibiting factor L-dopachrome isomerase L-dopachrome tautomerase Macrophage migration inhibitory factor (glycosylation-inhibiting factor) Macrophage migration inhibitory factor MIF MIF protein MIF_HUMAN MMIF Phenylpyruvate tautomerase |
Images
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Fig1:
Western blot analysis of MIF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate |
|
Fig2: Flow cytometric analysis of MIF was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-89, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.