MyoD1 Recombinant Rabbit Monoclonal Antibody [JM10-72]
cat.: ET1703-91
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JM10-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MyoD1 aa 36-80 / 320. |
| Positive control: | Hela cell lysates, Hela. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP FC |
1:1,000-1:5,000 1:10-1:50 1:50-1:100 |
| Uniprot #: | SwissProt: P15172 Human |
| Alternative names: | bHLHc1 Class C basic helix-loop-helix protein 1 MYF 3 Myf-3 MYF3 Myoblast determination protein 1 Myod 1 MYOD MYOD1 MYOD1_HUMAN Myogenic differentiation 1 Myogenic factor 3 Myogenic factor MYF 3 Myogenin D1 PUM |
Images
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Fig1: Western blot analysis of MyoD1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of MyoD1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-91, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.