CD26 Recombinant Rabbit Monoclonal Antibody [JM11-42]
cat.: ET1703-93
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JM11-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD26 aa 701-747 / 766 (Extracellular). |
| Positive control: | LoVo cell lysate, Caco-2 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, rat epididymis tissue. |
| Subcellular location: | Secreted, Cell membrane, Apical cell membrane, invadopodium membrane, lamellipodium membrane, Cell junction, Membrane raft. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P27487 Human | P28843 Mouse | P14740 Rat |
| Alternative names: | CD26 antigen ADA-binding protein ADABP ADCP 2 ADCP-2 ADCP2 Adenosine deaminase complexing protein 2 CD 26 CD26 CD26 antigen 3 Dipeptidyl peptidase 4 Dipeptidyl peptidase 4 soluble form Dipeptidyl peptidase IV Dipeptidyl peptidase IV membrane form Dipeptidyl peptidase IV soluble form Dipeptidyl peptidase, intestinal Dipeptidylpeptidase 4 Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) Dipeptidylpeptidase IV DPP 4 DPP IV DPP IV estoenzyme DPP4 DPP4_HUMAN DPPIV Intestinal dipeptidyl peptidase T cell activation antigen CD26 T-cell activation antigen CD26 TP 103 TP103 |
Images
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Fig1:
Western blot analysis of CD26 on different lysates with Rabbit anti-CD26 antibody (ET1703-93) at 1/5,000 dilution. Lane 1: LoVo cell lysate Lane 2: Caco-2 cell lysate Lane 3: Mouse thymus tissue lysate Lane 4: Rat thymus tissue lysate Lane 5: Mouse liver tissue lysate Lane 6: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 100/110 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-93) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
All lanes: Western blot analysis of CD26 with anti-CD26 antibody (ET1703-93) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: CD26 knockout Hela whole cell lysate (10 µg). ET1703-93 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-93, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.